# 586: THAUERA AROMATICA MEDIUM


## Main sol. 586

500	ml	**Solution A**	
500	ml	**Solution B**	
10	ml	**Trace element solution SL-10**	
5	ml	**Vitamin solution**	

1. Adjust solutions A and B to pH 7.2, autoclave separately and combine after cooling. Add 10 ml of sterile trace elements solution SL-10 (see below) and 5 ml vitamin solution (see below).


## Solution A

0.816	g	KH2PO4	
5.92	g	K2HPO4	
500	ml	Distilled water	


## Solution B

0.53	g	NH4Cl	
0.2	g	MgSO4 x 7 H2O	
2	g	KNO3	
0.025	g	CaCl2 x 2 H2O	
0.72	g	Na-benzoate	
500	ml	Distilled water	


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.


## Vitamin solution

50	mg	Vitamin B12	
50	mg	Pantothenic acid	
50	mg	Riboflavin	
10	mg	Pyridoxamine hydrochloride	
20	mg	Biotin	
20	mg	Folic acid	
25	mg	Nicotinic acid	
25	mg	Nicotine amide	
50	mg	alpha-lipoic acid	
50	mg	p-Aminobenzoic acid	
50	mg	Thiamine-HCl x 2 H2O	
1000	ml	Distilled water	

1. Stir for some hours, filter sterilize the solution.