# 592: THERMOMICROBIUM ROSEUM MEDIUM ## Main sol. 592 1 g Yeast extract 1 g Tryptone (BD 211705) 1.3 g (NH4)2SO4 (sublimed) 0.247 g MgSO4 x 7 H2O 0.28 g KH2PO4 0.074 g CaCl2 x 2 H2O 0.019 g FeCl3 x 6 H2O 1 ml **Trace elements** 999 ml Distilled water 20 g/l Agar, for solid medium 1. Adjust pH to 8.5. 2. Add 20.0 g/l agar for solid media and pour short slants with a long butt in screw-capped tubes. ## Trace elements 1.8 g MnCl2 x 4 H2O 4.4 g Na2B4O7 x 10 H2O 0.22 g ZnSO4 x 7 H2O 0.05 g CuCl2 x H2O 0.03 g Na2MoO4 x 2 H2O 0.03 g VOSO4 x 2 H2O 100 ml Distilled water 1. pH adjusted to 2.0 with H2SO4.