# 598: HALOVIBRIO VARIABILIS MEDIUM


## Main sol. 598

95	g	NaCl	
81	g	MgSO4 x 7 H2O	
1	g	KCl	
7.5	g	Yeast extract	
2.5	g	Proteose peptone	
10	ml	**Trace element solution SL-4**	
10	ml	**Vitamin solution**	
980	ml	Distilled water	

1. pH 7.5


## Vitamin solution

0.2	mg	Biotin	
0.2	mg	Folic acid	
1	mg	Pyridoxine hydrochloride	
0.5	mg	Thiamine-HCl x 2 H2O	
0.5	mg	Riboflavin	
0.5	mg	Nicotinic acid	
0.5	mg	Pantothenic acid	
0.01	mg	Vitamin B12	
0.5	mg	p-Aminobenzoic acid	
0.5	mg	Lipoic acid	
1000	ml	Distilled water	


## Trace element solution SL-4

0.5	g	Na2-EDTA	
0.2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.