# 61: THERMOANAEROBACTER MEDIUM


## Main sol. 61

10	g	Tryptone (BD BBL)	
2	g	Yeast extract	
0.5	ml	Sodium resazurin (0.1% w/v)	
0.2	g	FeSO4 x 7 H2O	
10	g	Sucrose	
0.2	g	Na2SO3	
0.08	g	Na2S2O3 x 5 H2O	
1000	ml	Distilled water	

1. Dissolve ingredients (except ferrous sulfate, sucrose, sulfite, and thiosulfate), adjust pH to 6.8 - 7.5, and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add ferrous sulfate, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sucrose, sulfite, and thiosulfate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. The stock solution of sulfite should be freshly prepared.