# 619: HYPHOMICROBIUM METHYLOVORUM MEDIUM


## Main sol. 619

10	ml	Methanol	
3	g	(NH4)2HPO4	
1	g	NaCl	
0.2	g	MgSO4 x 7 H2O	
10	mg	FeSO4 x 7 H2O	
5	mg	MnSO4 x 1 H2O	
5	ml	**Vitamin mixture**	
15	g	Agar	
1000	ml	Tap water	

1. Sterilize methanol by filtration, and add to other components sterilized by autoclaving.


## Vitamin mixture

0.2	mg	Biotin	
40	mg	Calcium pantothenate	
0.2	mg	Folic acid	
200	mg	Inositol	
40	mg	Niacin	
20	mg	p-Aminobenzoic acid	
40	mg	Pyridoxine hydrochloride	
40	mg	Riboflavin	
20	mg	Thiamine-HCl x 2 H2O	
100	mg	Choline	
2	µg	Vitamin B12	
1000	ml	Distilled water