# 630: MODIFIED THERMUS 162 MEDIUM


## Main sol. 630

2.5	g	Yeast extract	
2.5	g	Tryptone	
28	g	Agar	
100	mg	Nitrilotriacetic acid	
40	mg	CaSO4 x 2 H2O	
200	mg	MgCl2 x 6 H2O	
0.5	ml	Fe(III) citrate (0.01 M)	
0.5	ml	**Trace element solution**	
100	ml	**Phosphate buffer**	
900	ml	Distilled water	

1. Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min. Autoclave the phosphate buffer separately and then add to the medium.


## Phosphate buffer

5.44	g	KH2PO4	
43	g	Na2HPO4 x 12 H2O	
1000	ml	Distilled water	

1. Adjust pH to 7.2


## Trace element solution

12.8	g	Nitrilotriacetic acid	
1	g	FeCl2 x 4 H2O	
0.5	g	MnCl2 x 4 H2O	
0.3	g	CoCl2 x 4 H2O	
50	mg	CuCl2 x 2 H2O	
50	mg	Na2MoO4 x 2 H2O	
20	mg	H3BO3	
20	mg	NiCl2 x 6 H2O	
1000	ml	Distilled water	

1. Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 8 - 9 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.