# 63b: DESULFOVIBRIO MEDIUM (SEAWATER)


## Main sol. 63b

980	ml	**Solution A**	
10	ml	**Solution B**	
10	ml	**Solution C**	

1. Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Adjust pH of the complete medium to 6.8 - 7.0, if necessary.


## Solution A

0.5	g	K2HPO4	
1	g	NH4Cl	
1	g	Na2SO4	
0.1	g	CaCl2 x 2 H2O	
2	g	MgSO4 x 7 H2O	
2	g	Na-DL-lactate	
1	g	Yeast extract	
0.5	ml	Sodium resazurin (0.1% w/v)	
980	ml	Sea water (filtered and aged)	

1. Alternatively to filtered aged sea water bottled water from Biomaris GmbH (Bremen, Germany) can be used.


## Solution B

0.5	g	FeSO4 x 7 H2O	
10	ml	Distilled water	


## Solution C

0.1	g	Na-thioglycolate	
0.1	g	Ascorbic acid	
10	ml	Distilled water