# 63c: DESULFOVIBRIO MEDIUM (CHOLINE)


## Main sol. 63c

980	ml	**Solution A**	
10	ml	**Solution B**	
10	ml	**Solution C**	

1. Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Check medium pH after autoclaving and adjust to 6.8 - 7.0, if necessary.


## Solution A

0.5	g	K2HPO4	
1	g	NH4Cl	
1	g	Na2SO4	
0.1	g	CaCl2 x 2 H2O	
2	g	MgSO4 x 7 H2O	
5	g	Choline chloride (SIGMA)	
1	g	Yeast extract	
0.5	ml	Sodium resazurin (0.1% w/v)	
980	ml	Distilled water	


## Solution B

0.5	g	FeSO4 x 7 H2O	
10	ml	Distilled water	


## Solution C

0.1	g	Na-thioglycolate	
0.1	g	Ascorbic acid	
10	ml	Distilled water