# 645a: HEMIN MEDIUM FOR MYCOBACTERIUM


## Main sol. 645a

900	ml	Middlebrook 7H10 agar (Difco 0627)	
100	ml	OADC Enrichment (Difco 0722)	
60	μM	Haemin (solubilized with 1N NaOH)	

1. Middlebrook 7H10 (Difco 0627) with OADC Enrichment (Difco 0722) with 60 micromoles hemin. Hemin must be solubilized with 1N NAOH.