# 655: PYE MEDIUM


## Main sol. 655

1	g	K2HPO4	
0.2	g	MgSO4 x 7 H2O	
0.02	g	CaCl2 x 2 H2O	
0.2	g	Na2S2O3 x 5 H2O	
2.2	g	Na-pyruvate	
4	g	Yeast extract (Difco)	
1	g	(NH4)2SO4	
1	ml	**Trace element solution SL-6**	
1000	ml	Distilled water	

1. Adjust pH to 6.8.
2. Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Sterilize at 121°C for 15 min.


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water