# 659: M17 MEDIUM (modified)


## Main sol. 659

5	g	Casein peptone	
5	g	Soy peptone	
5	g	Meat peptone	
2.5	g	Yeast extract	
0.5	g	Ascorbic acid	
0.25	g	MgSO4 x 7 H2O	
9.5	g	Na2-ß-glycerolphosphate	
1000	ml	Distilled water	

1. After autoclaving add a lactose solution (sterilized by filtration) up to a concentration of 8.0 g/l, and 1.2 ml 1M CaCl2 x 2 H2O. Adjust pH to 7.15 ± 0.05.

8	g/l	Lactose (sterilized by filtration)	
1.2	ml	CaCl2 x 2 H2O