# 660: ALCALIGENES MEDIUM


## Main sol. 660

6.06	g	Tris	
4.68	g	NaCl	
1.49	g	KCl	
1.07	g	NH4Cl	
0.43	g	Na2SO4	
0.2	g	MgCl2 x 6 H2O	
0.03	g	CaCl2 x 2 H2O	
0.23	g	Na2HPO4 x 12 H2O	
5	mg	Fe(NH4)citrate	
1	ml	**Trace element solution SL-7**	
988.5	ml	Distilled water	

1. After autoclaving add 2.5 ml 1.0 M CuSO4 and 10 ml of a 40% Na-succinate solution, both sterilized by filtration.

2.5	ml	CuSO4 (1 M, sterilized by filtration)	
10	ml	Sodium succinate (40 %, sterilized by filtration)	


## Trace element solution SL-7

1	ml	HCl (25%)	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
60	mg	H3BO3	
200	mg	CoCl2 x 6 H2O	
20	mg	CuCl2 x 2 H2O	
20	mg	NiCl2 x 6 H2O	
40	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water