# 665: SULFOBACILLUS MEDIUM ## Main sol. 665 700 ml **Solution A** 301 ml **Solution B** 20 ml **Solution C** 1. After autoclaving, combine the three solutions. Medium pH 1.9 - 2.4. ## Solution A 3 g (NH4)2SO4 0.1 g KCl 0.5 g K2HPO4 0.5 g MgSO4 x 7 H2O 0.01 g Ca(NO3)2 700 ml Distilled water 1. Adjust pH to 2.0 - 2.2 with sulfuric acid. ## Solution B 44.2 g FeSO4 x 7 H2O 300 ml Distilled water 1 ml H2SO4, 10 N 1. (sterilize by filtration or handle under N2 before autoclaving to prevent oxidation of the iron) ## Solution C 20 ml Yeast extract (1% w/v in water)