# 68: HALOTHIOBACILLUS NEAPOLITANUS MEDIUM


## Main sol. 68

4	g	KH2PO4	
4	g	K2HPO4	
0.8	g	MgSO4 x 7 H2O	
0.4	g	NH4Cl	
5	ml	**Trace element solution (Vishniac & Santer, 1957)**	
0.5	ml	Bromocresol purple (saturated aqueous solution)	
15	g	Agar (Bacto), for solid medium (optional)	
10	g	Na2S2O3 x 5 H2O	
1000	ml	Distilled water	

1. Dissolve ingredients except thiosulfate, dispense into suitable cultivation vessels and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution. Adjust pH of complete medium to 6.6 - 7.0.


## Trace element solution (Vishniac & Santer, 1957)

50	g	Na2-EDTA	
22	g	ZnSO4 x 7 H2O	
5.54	g	CaCl2 x 2 H2O	
5.06	g	MnCl2 x 4 H2O	
5	g	FeSO4 x 7 H2O	
1.1	g	(NH4)6Mo7O24 x 4 H2O	
1.57	g	CuSO4 x 5 H2O	
1.61	g	CoCl2 x 6 H2O	
1000	ml	Distilled water	

1. Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.