# 69: STARKEYA NOVELLA MEDIUM


## Main sol. 69

900	ml	**Solution A**	
50	ml	**Solution B**	
5	ml	**Solution C**	
50	ml	**Solution D**	

1. Solutions A, B, C and D are sterilized separately by autoclaving and mixed when cooled to room temperature. Adjust pH of complete medium to 8.5 with sterile 0.5 N NaOH.


## Solution A

10.6	g	Na2HPO4 x 12 H2O	
1.5	g	KH2PO4	
0.3	g	NH4Cl	
0.3	g	Yeast extract	
2	ml	Phenol red (0.1% w/v)	
15	g	Agar (Bacto), for solid medium (optional)	
900	ml	Distilled water	


## Solution B

0.1	g	MgSO4 x 7 H2O	
50	ml	Distilled water	


## Solution C

5	ml	**Trace element solution (Vishniac & Santer, 1957)**	


## Solution D

5	g	Na2S2O3 x 5 H2O	
50	ml	Distilled water	


## Trace element solution (Vishniac & Santer, 1957)

50	g	Na2-EDTA	
22	g	ZnSO4 x 7 H2O	
5.54	g	CaCl2 x 2 H2O	
5.06	g	MnCl2 x 4 H2O	
5	g	FeSO4 x 7 H2O	
1.1	g	(NH4)6Mo7O24 x 4 H2O	
1.57	g	CuSO4 x 5 H2O	
1.61	g	CoCl2 x 6 H2O	
1000	ml	Distilled water	

1. Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.