# 693: COLUMBIA BLOOD MEDIUM


## Main sol. 693

1000	ml	Columbia agar base	
5	%	Defibrinated sheep blood	

1. Columbia agar base supplemented with 5% defibrinated sheep blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below).
2. ATTENTION: organisms, for which only M 693 is mentioned in the catalogue entry, do not grow in one of the media listed below! Those are meant to rehydrate the freeze-dried pellets, not for cultivation.


## 545. TRYPTONE SOYA BROTH (TSB)

17	g	Casein peptone	
3	g	Soy peptone	
2.5	g	D(+)-Glucose	
5	g	NaCl	
2.5	g	K2HPO4	
1000	ml	Distilled water	

1. Adjust pH to 7.3.
2. The same as Caso Bouillon (Merck or Oxoid, Germany).


## 215. BHI MEDIUM

37	g	Brain heart infusion (Difco)	
1000	ml	Distilled water	


## 1. NUTRIENT BROTH

5	g	Peptone	
3	g	Meat extract	
1000	ml	Distilled water	

1. Adjust pH to 7.0.