# 699: TRIPLE-SUGAR-IRON MEDIUM (Difco)


## Main sol. 699

3	g	Bacto beef extract	
3	g	Yeast extract (Bacto )	
15	g	Bacto peptone	
1	g	Dextrose	
10	g	Lactose	
10	g	Sucrose	
0.2	g	FeSO4	
5	g	NaCl	
0.3	g	Na2S2O3	
0.024	g	Phenol red	
12	g	Agar, if required	
1000	ml	Distilled water	

1. pH 7.4