# 70: ACIDITHIOBACILLUS FERROOXIDANS MEDIUM


## Main sol. 70

0.4	g	KH2PO4	
0.4	g	MgSO4 x 7 H2O	
0.4	g	(NH4)2SO4	
33.3	g	FeSO4 x 7 H2O	
1000	ml	H2SO4 (0.1 N)	

1. Adjust pH to 1.8 with 2N sulfuric acid. Sterilize by autoclaving for 15 min at 121°C.