# 748: CENS MEDIUM


## Main sol. 748

0.9	g	K2HPO4	
0.6	g	KH2PO4	
1	g	NH4Cl	
5	mg	EDTA	
0.2	g	MgSO4 x 7 H2O	
75	mg	CaCl2 x 2 H2O	
1	ml	**True Blue Trace Elements**	
2	ml	**Chelated Iron solution**	
20	µg	Vitamin B12	
15	µg	Biotin	
0.5	g	Na2S2O3 x 5 H2O	
4	g	Soytone (Difco 0436)	
2.2	g	Na-pyruvate (filter-sterilized)	
1000	ml	Distilled water	


## True Blue Trace Elements

2.5	g	EDTA	
0.2	g	MnCl2 x 4 H2O	
0.1	g	H3BO3	
0.1	g	Na2MoO4 x 2 H2O	
50	mg	ZnCl2	
50	mg	NiCl2 x 6 H2O	
20	mg	CoCl2 x 2 H2O	
10	mg	CuCl2 x 2 H2O	
5	mg	Na2SeO3	
5	mg	NaVO3 x n H2O (meta)	
250	ml	Distilled water	

1. Dissolve chemicals one at a time in approximately 200.0 ml distilled water. Bring final volume to 250.0 ml with additional water.


## Chelated Iron solution

1	g	FeCl2 x 4 H2O	
2	g	EDTA	
3	ml	HCl (conc.)	
900	ml	Distilled water