# 762: HALOMONAS DESIDERATA MEDIUM


## Main sol. 762

5	g	Glucose	
0.2	g	MgCl2 x 6 H2O	
1	g	KH2PO4	
2	g	KNO3	
5.4	g	Na2CO3	
4.2	g	NaHCO3	
1000	ml	Distilled water	

1. Prepare the medium without Na2CO3 and NaHCO3 in 900 ml distilled water and autoclave. Prepare the Na2CO3 and NaHCO3 in 100 ml water, sterilise separately and add to the cooled medium. Final pH 9.5-10.0.