# 772: PARACOCCUS ALCALIPHILUS MEDIUM


## Main sol. 772

3	g	(NH4)2SO4	
1.4	g	KH2PO4	
3	g	Na2HPO4	
0.2	g	MgSO4 x 7 H2O	
30	mg	Fe(III) citrate	
30	mg	CaCl2 x 2 H2O	
5	mg	MnCl2 x 4 H2O	
5	mg	ZnSO4 x 7 H2O	
0.5	mg	CuSO4 x 2 H2O	
0.4	mg	Thiamine-HCl x 2 H2O	
1000	ml	Distilled water	
10	ml	Methanol (filter-sterilized)	

1. Thiamine hydrochloride may be replaced by 2 g/l yeast extract. The pH of the medium is adjusted to pH 9.0 with filter sterilised 10% Na2CO3. 10 ml sterile methanol (filter sterilised, or autoclaved in crimp top sealed bottles) is added to the sterilised medium.