# 791: PORPHYROBACTER TEPIDARIUS MEDIUM


## Main sol. 791

0.5	g/l	Na glutamate	
0.5	g/l	Sodium succinate	
0.5	g/l	Sodium acetate	
0.5	g/l	Yeast extract	
0.5	g/l	Casamino acids	
0.5	g/l	(NH4)2SO4	
0.5	g/l	Sodium thiosulphate	
5	ml	**Basal salts solution**	
5	ml	**Phosphate solution**	
1	ml	** Vitamin solution**	
989	ml	Distilled water	

1. Final pH 7.5
2. Add the vitamin solution, aseptically to the cooled, autoclaved medium.


## Basal salts solution

4.53	g/l	Na3-EDTA	
1.11	g/l	FeSO4 x 7 H2O	
24.65	g/l	MgSO4 x 7 H2O	
2.94	g/l	CaCl2 x 2 H2O	
23.4	g/l	NaCl	
112	mg/l	MnSO4 x 4 H2O	
29	mg/l	ZnSO4 x 7 H2O	
29	mg/l	Co(NO3)2 x 6 H2O	
25	mg/l	CuSO4 x 5 H2O	
24	mg/l	Na2Mo4 x 2 H2O	
31	mg/l	H3BO3	
1000	ml	Distilled water	


## Phosphate solution

75	g	KH2PO4	
78	g	K2HPO4	
1000	ml	Distilled water	


##  Vitamin solution

100	mg	Nicotinic acid	
100	mg	Thiamine	
5	mg	Biotin	
50	mg	p-Aminobenzoic acid	
1	mg	Vitamin B12	
50	mg	Calcium pantothenate	
50	mg	Pyridoxine hydrochloride	
50	mg	Folic acid	
200	mg	Na3-EDTA	
100	ml	Distilled water	

1. Filter sterilize.