# 807: HALOANAEROBIUM ALCALIPHILUM MEDIUM


## Main sol. 807

17	g	MgSO4 x 7 H2O	
100	g	NaCl	
50	ml	**Solution A**	
50	ml	**Solution B**	
10	ml	**Trace element solution SL-10**	
1	mg	Resazurin	
10	g	Yeast extract	
10	g	Trypticase	
5	g	Glucose	
0.25	g	Na2S x 9 H2O	
4.1	g	NaHCO3	
0.5	g	L-Cysteine HCl x H2O	
890	ml	Distilled water	

1. pH 7.0
2. Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v) and add the NaHCO3 and cysteine hydrochloride. Autoclave the medium and add from sterile stock solutions yeast extract, trypticase, glucose, and Na2S x 9 H2O. The final pH should be 7.0.


## Solution A

6	g/l	K2HPO4	


## Solution B

6	g	KH2PO4	
6	g	(NH4)2SO4	
12	g	NaCl	
2.6	g	MgSO4 x 7 H2O	
2.5	g	NH4Cl	
0.28	g	CaCl2 x 2 H2O	
0.28	g	K2HPO4	


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.