# 812: SULFOBACILLUS DISULFIDOOXIDANS MEDIUM


## Main sol. 812

3	g	(NH4)2SO4	
0.1	g	KCl	
0.5	g	KH2PO4	
0.5	g	MgSO4 x 7 H2O	
0.1	g	Ca(NO3)2 x 4 H2O	
0.1	g	Yeast extract	
1000	ml	Distilled water	
10	ml	Glutathione (10% w/v)	

1. Adjust pH to 2.25 prior to autoclaving. Before use add per liter 10 ml of a filter sterilized 10% (w/v) glutathione solution.