# 815: DESULFOHALOTOMACULUM MEDIUM


## Main sol. 815

3	g	Na2SO4	
6	g	CaCl2 x 2 H2O	
40	g	NaCl	
2	g	KCl	
0.3	g	NH4Cl	
0.2	g	KH2PO4	
8	g	MgCl2 x 6 H2O	
0.1	g	SrCl2 x 6 H2O	
1	ml	**Trace element solution SL-11**	
3	g	MOPS	
1	g	Yeast extract	
0.5	ml	Sodium resazurin (0.1% w/v)	
100	g	Iron powder (or nails)	
3.6	g	Na-L-lactate	
0.5	g	Na2CO3	
1000	ml	Distilled water	

1. Dissolve ingredients except iron, lactate and carbonate, adjust pH to 6.0 with NaOH and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing 1 - 2 g iron powder or an iron nail. Autoclave for 30 min at 105°C. Before use add lactate from a sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Final medium pH should be 7.2.


## Trace element solution SL-11

5.2	g	Na2-EDTA x 2 H2O	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.