# 830b: MEDIUM R2A FOR DENITRIFYING BACTERIA


## Main sol. 830b

0.5	g	Yeast extract	
0.5	g	Proteose peptone (Difco no. 3)	
0.5	g	Casamino acids	
0.5	g	Glucose	
0.5	g	Starch (soluble)	
0.3	g	Na-pyruvate	
0.3	g	K2HPO4	
0.05	g	MgSO4 x 7 H2O	
0.5	g	KNO3	
10	ml	**Trace element solution SL-10**	
1000	ml	Distilled water	

1. Prepare liquid medium under anaerobic conditions (N2).


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.