# 836: DESULFITOBACTERIUM PCP-1 MEDIUM


## Main sol. 836

0.27	g	KH2PO4	
0.35	g	K2HPO4	
0.53	g	NH4Cl	
0.1	g	MgCl2 x 6 H2O	
2	ml	FeCl2 x 4 H2O (0.1% w/v in 0.2 N HCl)	
0.07	g	CaCl2 x 2 H2O	
1	ml	**Trace element solution SL-10**	
1	g	Yeast extract	
6.05	g	Na-pyruvate	
0.5	ml	Sodium resazurin (0.1% w/v)	
0.5	g	Na2CO3	
0.63	g	Na2SO3	
0.05	g	Na2S x 9 H2O	
1000	ml	Distilled water	

1. Dissolve ingredients (except carbonate, sulfite and sulfide), adjust pH of solution to 7.0 and sparge with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add sulfite and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The stock solution of sulfite should by freshly prepared and sterilized by filtration. Prior to use check pH of complete medium and adjust to 7.5, if necessary.


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.