# 849: AE MEDIUM


## Main sol. 849

5	g	Glucose	
3	g	Yeast extract	
4	g	Peptone	
30	ml	Absolute ethanol	
30	ml	Glacial acetic acid	
9	g	Agar	
940	ml	Distilled water	

1. Both liquid and solid media are prepared by autoclaving the medium without the addition of glacial acetic acid and absolute ethanol. Glacial acetic acid and absolute ethanol are sterilised either by filtration (use Teflon filters) or they may be autoclaved in completely closed screw cap glass bottles sealed with a Teflon coated septum. A double layer system das not appear to be essential for the growth of this organism, however, plates should be incubated in sealed containers to keep the humidity high (> 90%).