# 864: HALOTHIOBACILLUS MEDIUM


## Main sol. 864

29	g	NaCl	
1	g	(NH4)2SO4	
1.5	g	MgSO4 x 7 H2O	
0.42	g	CaCl2 x 2 H2O	
0.7	g	KCl	
1	ml	**Trace element solution SL-11**	
4	ml	Bromothymol blue (0.1% w/v)	
0.5	g	K2HPO4	
5	g	Na2S2O3 x 5 H2O	
1000	ml	Distilled water	

1. Dissolve ingredients except hydrogenphosphate and thiosulfate, adjust pH to 6.5, then dispense medium into culture vessels and autoclave. Add hydrogenphosphate and thiosulfate from sterile stock solutions. Adjust pH of complete medium to 6.5 - 7.0.


## Trace element solution SL-11

5.2	g	Na2-EDTA x 2 H2O	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.