# 874: FERROPLASMA ACIDIPHILUM MEDIUM


## Main sol. 874

930	ml	**Solution A**	
50	ml	**Solution B**	
10	ml	**Solution C**	
10	ml	**Solution D**	

1. Add solutions B and C to solution A, adjust pH of the combined solution to 1.6 - 1.8 with H2SO4, then sterilize using membrane filter and dispense in sterile culture vessels. Prior to use, add the appropriate volume of solution D sterilized by autoclaving.


## Solution A

0.4	g	MgSO4 x 7 H2O	
0.2	g	(NH4)2SO4	
0.1	g	KCl	
0.1	g	K2HPO4	
930	ml	Distilled water	


## Solution B

25	g	FeSO4 x 7 H2O	
40	ml	H2SO4 (1 N)	
10	ml	Distilled water	


## Solution C

10	ml	**Trace element solution**	


## Solution D

0.2	g	Yeast extract (OXOID)	
10	ml	Distilled water	


## Trace element solution

1.93	g	FeCl3 x 6 H2O	
0.18	g	MnCl2 x 4 H2O	
0.45	g	Na2B4O7 x 10 H2O	
22	mg	ZnSO4 x 7 H2O	
5	mg	CuCl2 x 2 H2O	
3	mg	Na2MoO4 x 2 H2O	
3.8	mg	VOSO4 x 5 H2O	
2	mg	CoSO4 x 7 H2O	
1000	ml	Distilled water