# 884: TWEEN 80-AGAR ## Main sol. 884 900 ml **Solution A** 100 ml **Solution B** 1. Autoclave solutions A and B separately and combine after cooling to approximately 50°C. ## Solution A 10 g Peptone 5 g NaCl 0.1 g CaCl2 x 2 H2O 15 g Agar 900 ml Distilled water ## Solution B 10 g Tween 80 100 ml Distilled water