# 886: HELIORESTIS MEDIUM


## Main sol. 886

0.5	g	KH2PO4	
0.5	g	NH4Cl	
0.5	g	NaCl	
0.6	g	MgCl2 x 6 H2O	
0.1	g	CaCl2	
2.5	g	Na2CO3	
2.5	g	NaHCO3	
1	g	Na-acetate	
0.5	g	Yeast extract	
0.4	g	Na2S x 9 H2O	
20	µg	Vitamin B12	
20	µg	Biotin	
1	ml	**Trace element solution SL-6**	
1000	ml	Distilled water	
0.002	%	Resazurin	

1. Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Sterilize at 121°C for 15 min.
2. Prepare NaHCO3, Na2CO3 and sulfide as separate sterile stock solution, Vitamin B12 and Biotin as filter-sterilized stock solution and add to sterile medium after autoclaving. Adjust pH to 9 - 9.5 .
3. To control anoxic conditions, add resazurin to a final concentration of 0.002%.


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water