# 923: ALKALIBACTERIUM OLIVAPOVLITICUS


## Main sol. 923

10	g	Na glutamate (Sigma)	
5	g	Yeast extract (Oxoid)	
10	g	Na2CO3	
0.15	g	K2HPO4	
0.025	g	MgSO4 x 7 H2O	
1	g	(NH4)2SO4	
1000	ml	Distilled water	
20	g	Agar, for solid medium	

1. We recommend that liquid media be made by autoclaving the Na2CO3 separately, otherwise the high pH will cause ammonia to be lost. When making media solidified with agar (20 g/l) the agar should also not be autoclaved with the Na2CO3 otherwise the agar will be hydrolysed. When mixing the ingredients for solid media cool all components to about 55°C otherwise hydrolysis of the agar may occur.