# 92a: MODIFIED MEDIUM 92


## Main sol. 92a

990	ml	**Main sol. 92**	
10	ml	**Vitamin solution**	

1. Prepare medium 92 and subsequently add vitamins. Adjust pH to 7.0 - 7.2


## Vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine-HCl x 2 H2O	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
1	mg	p-Aminobenzoic acid	
0.01	mg	Vitamin B12	
1000	ml	Distilled water	


## Main sol. 92

30	g	Trypticase soy broth	
3	g	Yeast extract	
15	g	Agar, if necessary	
1000	ml	Distilled water	

1. Adjust pH to 7.0 - 7.2.