# 939: METHYLOTROPHIC ARTHROBACTER AND HYPHOMICROBIUM MEDIUM


## Main sol. 939

7.9	g	Na2HPO4 x 2 H2O	
1.5	g	KH2PO4	
0.8	g	NH4Cl	
0.1	g	MgSO4 x 7 H2O	
10	ml	**Trace elements solution**	
1000	ml	Distilled water	
10	ml	Methanol	

1. Final pH 7.2-7.5
2. After autoclaving add 10 ml methanol to 1 litre of the sterile Mineral Salts Solution.


## Trace elements solution

50	g	EDTA	
1	g	ZnSO4 x 7 H2O	
7.34	g	CaCl2 x 2 H2O	
2.5	g	MnCl2 x 4 H2O	
0.5	g	CoCl2 x 6 H2O	
0.5	g	(NH4)6Mo7O24	
5	g	FeSO4 x 7 H2O	
0.2	g	CuSO4 x 5 H2O	
1000	ml	Distilled water	

1. Dissolve the EDTA in about 400 ml of water, then add 9 g NaOH. Dissolve each of the salts individually in about 40-50 ml of water and add them to the EDTA-NaOH solution. Adjust the final pH of the solution to pH 6.0 with 1M NaOH (about 24 ml). Make up to 1 litre with distilled water, store in the dark and do not autoclave the stock solution before it is added to the medium.