# 944: PSEUDOMONAS CHLOROTIDISMUTANS MEDIUM


## Main sol. 944

900	ml	**Solution A**	
50	ml	**Solution B**	
50	ml	**Solution C**	
1	ml	**Vitamin solution**	

1. All solutions are prepared anaerobically under N2 and autoclaved. After cooling, the solutions are combined under N2 gas. Add 1 ml/l vitamin solution. pH should be 8.5 without further adjustment.


## Solution A

0.41	g	KH2PO4	
0.53	g	Na2HPO4	
1.06	g	NaClO3	
2.72	g	Na-acetate x 3 H2O	
0.5	mg	Resazurin	
1	ml	**Trace element solution SL-10**	
10	ml	**Selenite-tungstate-molybdate solution**	
900	ml	Distilled water	


## Solution B

0.11	g	CaCl2	
0.1	g	MgCl2	
50	ml	Distilled water	


## Solution C

0.44	g	NH4HCO3	
3.73	g	NaHCO3	
0.5	g	Na2S x 9 H2O	
50	ml	Distilled water	


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.


## Selenite-tungstate-molybdate solution

500	mg	NaOH	
3	mg	Na2SeO3 x 5 H2O	
3	mg	Na2WO4 x 2 H2O	
3	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## Vitamin solution

50	mg	Vitamin B12	
50	mg	Pantothenic acid	
50	mg	Riboflavin	
10	mg	Pyridoxamine hydrochloride	
20	mg	Biotin	
20	mg	Folic acid	
25	mg	Nicotinic acid	
25	mg	Nicotine amide	
50	mg	alpha-lipoic acid	
50	mg	p-Aminobenzoic acid	
50	mg	Thiamine-HCl x 2 H2O	
1000	ml	Distilled water	

1. Stir for some hours, filter sterilize the solution.