# 959a: MARINITOGA LITORALIS MEDIUM


## Main sol. 959a

26	g	NaCl	
0.5	g	MgCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
1	g	NH4Cl	
0.2	g	KCl	
3.4	g	PIPES (SIGMA)	
0.35	g	K2HPO4	
0.35	g	KH2PO4	
2.72	g	Na-acetate x 3 H2O	
2	g	Yeast extract (OXOID)	
2	g	Trypticase peptone (BD BBL)	
0.5	ml	Sodium resazurin (0.1% w/v)	
3.5	g	Maltose	
0.5	g	Na2S x 9 H2O	
1000	ml	Distilled water	

1. Dissolve ingredients except maltose and sulfide, adjust pH to 5.8 - 6.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add maltose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. The pH of the complete medium should be 6.0.