# 964: CAMINICELLA MEDIUM


## Main sol. 964

30	g	Sea Salt (SIGMA)	
6.05	g	PIPES buffer (SIGMA)	
5	g	D-Glucose	
0.5	ml	Sodium resazurin (0.1% w/v)	
12	g	Sulfur (powdered)	
1	g	Peptone (BD Bacto)	
0.5	g	Yeast extract (BD Bacto)	
0.5	g	Na2S x 9 H2O	
1000	ml	Distilled water	

1. Dissolve ingredients (except sulfur, peptone, yeast extract, and sulfide), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 7.2 - 7.4, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and autoclave at <b>110°C</b> for 20 min. Add peptone, yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The pH of the complete medium should be 7.5.