# 976: METHYLOPHAGA ALCALICA MEDIUM


## Main sol. 976

1	g	KH2PO4	
1	g	KNO3	
0.22	g	MgSO4 x 7 H2O	
30	g	NaCl	
5	g	Na2CO3	
1	ml	**Trace elements solution**	
1000	ml	Distilled water	
10	ml	Methanol	

1. Final pH 9.5
2. Prepare the medium without the Na2CO3, which can be sterilised separately by autoclaving. 10 ml/l sterile methanol is added to the cooled medium. When preparing liquid media cool the mineral salts solution and Na2CO3 to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salts solution and autoclave. Cool the Na2CO3 stock solution and agar to 50-55°C before mixing.


## Trace elements solution

30	mg	Ferric citrate	
30	mg	CaCl2 x 2 H2O	
5	mg	MgCl2 x 4 H2O	
5	mg	ZnSO4 x 7 H2O	
0.5	g	CuSO4 x 5 H2O	
1000	ml	Distilled water	
2	%	Agar, for solid medium