# 98: RHIZOBIUM MEDIUM


## Main sol. 98

1	g	Yeast extract	
10	g	Mannitol	
15	g	Agar	
200	ml	**Soil extract**	
800	ml	Distilled water	

1. Adjust pH to 7.0.


## Soil extract

80	g	air-dried garden soil	
0.2	g	Na2CO3	
200	ml	Distilled water	

1. Autoclave soil suspension for one hour at 121°C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2.