# 993a: NESTERENKONIA MODIFIED MEDIUM


## Main sol. 993a

1000	ml	**Main sol. 993**	
0.5	%	Yeast extract	
10	%	MgCl2 x 6 H2O	

1. Use medium 993 supplemented with Yeast extract (0.5%) and MgCl2 x 6 H20 (10%).


## Main sol. 993

1	g	L-Asparagine (anhydrous basis)	
10	g	Glycerol	
1	g	K2HPO4 (anhydrous basis)	
1000	ml	Distilled water	
1	ml	**Trace element solution**	
20	g	Agar	

1. The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes.
2. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.


## Trace element solution

0.1	g	FeSO4 x 7 H2O	
0.1	g	MnCl2 x 4 H2O	
0.1	g	ZnSO4 x 7 H2O	
100	ml	Distilled water