# C110: ASN-III


## Mail sol. C110

25	g	NaCl	
3.5	g	MgSO4 x 7 H2O	
2	g	MgCl2 x 6 H2O	
0.5	g	CaCl2 x 2 H2O	
0.5	g	KCl	
3	mg	Citric acid	
3	mg	Ferric ammonium citrate	
0.5	mg	EDTA	
1	ml	**A-5 Trace Metals**	
0.75	g	NaNO3	
0.75	g	K2HPO4 x 3 H2O	
0.02	g	Na2CO3	
10	mg	Vitamin B12	
10	g	Agar (Noble)	
1000	ml	Distilled water	

1. Adjust pH to 7.3. Filter-sterilize and dispense into appropriate vessel.


## A-5 Trace Metals

2.86	g	H3BO3	
1.81	g	MnCl2 x 4 H2O	
0.222	g	ZnSO4 x 7 H2O	
0.039	g	Na2MoO4 x 2 H2O	
0.079	g	CuSO4 x 5 H2O	
0.049	g	Co(NO3)2 x 6 H2O	
1000	ml	Distilled water