# C27: EG:JM


## Mail sol. C27

500	ml	**EG (Euglena gracilis Medium)**	
500	ml	**JM (Jaworski´s Medium)**	

1. 1:1 mixture. See separate recipes. Mix then autoclave at 15 psi for 15 minutes.


## EG (Euglena gracilis Medium)

1	g	Sodium acetate x 3 H2O	
1	g	Lab Lemco powder (Oxoid L29)	
2	g	Tryptone (Oxoid L42)	
2	g	Yeast extract (Oxoid L21)	
10	ml	CaCl2 (1 g/l stock solution)	
1000	ml	Deionized water	
15	g	Agar (Oxoid L11), for solid medium	

1. Add constituents above and make up to 1 litre with deionized water. For agar add 15 g per
litre Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.


## JM (Jaworski´s Medium)

1	ml	Ca(NO3)2 x 4 H2O (4.0 g/200 ml stock solution)	
1	ml	KH2PO4 (2.48 g/200 ml stock solution)	
1	ml	MgSO4 x 7 H2O (10.0 g/200 ml stock solution)	
1	ml	NaHCO3 (3.18 g/200 ml stock solution)	
1	ml	**Solution 1**	
1	ml	**Solution 2**	
1	ml	**Solution 3**	
1	ml	NaNO3 (16.0 g/200 ml stock solution)	
1	ml	Na2HPO4 x 12 H2O (7.2 g/200 ml stock solution)	
1000	ml	Deionized water	
15	g	Agar (Oxoid L11), for solid medium	

1. Make up to 1 litre with deionized water.  For agar, add 15.0 g per litre of Bacterial Agar (Oxoid L11).  Autoclave at 15 psi for 15 minutes.


## Solution 1

0.45	g	FeNa-EDTA	
0.45	g	Na2-EDTA	


## Solution 2

0.496	g	H3BO3	
0.278	g	MnCl2 x 4 H2O	
0.2	g	(NH4)6Mo7O24 x 4 H2O	


## Solution 3

0.008	g	Cyanocobalamine	
0.008	g	Thiamine HCl	
0.008	g	Biotine