# C34: f/2


## Mail sol. C34

1	ml	NaNO3 (15 g/200 ml stock solution)	
1	ml	NaH2PO4 x 2 H2O (1.13 g/200 ml stock solution)	
0.1	ml	**Trace elements (x10 concentration)**	
1	ml	**Vitamin mix**	
1000	ml	Natural sea water (filtered)	
15	g	Agar, for solid medium	

1. Make up to 1 litre with filtered natural seawater. Adjust pH to 8.0 with 1M NaOH or 1 M HCl
prior to autoclaving. For agar add 15g per litre Bacteriological Agar. Autoclave at 15 psi for 15
minutes.


## Trace elements (x10 concentration)

8.32	g	Na2-EDTA	
6.3	g	FeCl3 x 6 H2O	
0.02	g	CuSO4 x 5 H2O	
0.044	g	ZnSO4 x 7 H2O	
0.02	g	CoCl2 x 6 H2O	
0.36	g	MnCl2 x 4 H2O	
0.012	g	Na2MoO4 x 2 H2O	


## Vitamin mix

1. First make primary stocks of Cyanocobalamin and Biotin.

1	ml	Cyanocobalamine (0.01 g/100 ml stock solution)	
1	ml	Biotin (0.01 g/100 ml stock solution)	

2. Dispense any excess primary stocks into 1 ml aliquots and freeze.

0.02	g	Thiamine HCl