# C40: JM


## Mail sol. C40

1	ml	Ca(NO3)2 x 4 H2O (4.0 g/200 ml stock solution)	
1	ml	KH2PO4 (2.48 g/200 ml stock solution)	
1	ml	MgSO4 x 7 H2O (10.0 g/200 ml stock solution)	
1	ml	NaHCO3 (3.18 g/200 ml stock solution)	
1	ml	**Solution 1**	
1	ml	**Solution 2**	
1	ml	**Solution 3**	
1	ml	NaNO3 (16.0 g/200 ml stock solution)	
1	ml	Na2HPO4 x 12 H2O (7.2 g/200 ml stock solution)	
1000	ml	Deionized water	
15	g	Agar, for solid medium	

1. Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacteriological Agar
(Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.


## Solution 1

0.45	g	FeNa-EDTA	
0.45	g	Na2-EDTA	


## Solution 2

0.496	g	H3BO3	
0.278	g	MnCl2 x 4 H2O	
0.2	g	(NH4)6Mo7O24 x 4 H2O	


## Solution 3

0.008	g	Cyanocobalamine	
0.008	g	Thiamine HCl	
0.008	g	Biotine