# C88: R75S:NSW


## Mail sol. C88

600	ml	**Mail sol. C87**	
300	ml	**NSW (Natural Seawater)**	

1. 2:1 mixture
2. See separate recipes below.  Autoclave separately.  Mix aseptically when cool.


## Mail sol. C87

750	ml	Natural sea water (filtered)	
250	ml	Deionized water	
1	g	Rye grass (dried, milled)	

1. Bring 75% seawater to the boil, add the powdered rye grass and keep boiling for 5 
minutes.  Allow to cool and  restore volume to 1 litre with deionized water.  Filter 
through Whatman GF/C paper.  Autoclave at 15 psi for 15 minutes.


## NSW (Natural Seawater)

1000	ml	Natural sea water (filtered)	

1. Filter natural seawater through GF/C filters and autoclave at 15 psi for 15 minutes.