# C99: S/W


## Mail sol. C99

x	g	Soil (air-dried, sieved, garden loam)	

1. Put a layer about 1 cm deep of air-dried, sieved good calcareous garden loam into a
test tube or jar. (The use of mud from rivers or ponds is rarely satisfactory.)
Carefully add deionised water to a depth of 7 to 10 cm, plug or cover, and steam
for one hour or autoclave for 15 minutes at 15 psi (longer for larger vessels) on
each of 2 consecutive days; further sterilisation is not needed. Allow to stand for a
further day before inoculating, when the pH should be between 7.0 - 8.0.