# J1006: HALOPHILIC SOB MEDIUM


## Main sol. J1006

1	g	(NH4)2SO4	
1	g	MgSO4 x 7 H2O	
0.3	g	CaCl2 x 2 H2O	
0.6	g	KCl	
100	g	NaCl	
1000	ml	Distilled water	

1. Mix components thoroughly and autoclave. Aseptically add the following solutions (autoclaved or *filter-sterilized): 

20	ml	Na2S2O3 (1 M)	
3	ml	K2HPO4 (1 M)	
5	ml	NH4Cl (1 M)	
1	ml	EDTA (0.5 M)	
10	ml	NaHCO3 (*, 1 M)	
0.06	ml	Trace element solution (see below, *)	
0.06	ml	**Trace vitamins** (*)	
6	�l	**Vitamin B12 solution 0.05 g/L** (*)	

2. Adjust pH to 7.5.


## Trace metal solution

1	ml	HCl (32%)	
2	g	FeCl2 x 4 H2O	
0.05	g	H3BO3	
0.05	g	ZnCl2	
0.03	g	CuCl2 x 2 H2O	
0.05	g	MnCl2 x 4 H2O	
0.05	g	Na2MoO4 x 2 H2O	
0.05	g	AlCl3	
0.2	g	CoCl2 x 6 H2O	
0.05	g	NiCl2 x 6 H2O	
0.05	g	Na2SeO3 x 5 H2O	
0.05	g	Na2WO4 x 2 H2O	
1000	ml	Distilled water	


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water	


## Vitamin B12 solution 0.05 g/L

5	mg	Vitamin B12	
100	ml	Distilled water