# J1024: THERMUS 162 MEDIUM


## Main sol. J1024

1	g	Yeast extract (BD-Difco)	
1	g	Tryptone (BD-Difco)	
0.1	g	Nitrilotriacetic acid	
0.04	g	CaSO4 x 2 H2O	
0.2	g	MgCl2 x 6 H2O	
0.5	ml	Iron(III) citrate (0.01 M)	
0.5	ml	**Nitsch's trace elements**	
900	ml	Distilled water	

1. Adjust pH to 7.2 with NaOH. For preparation of solid medium, add 28.0 g/L agar. After autoclaving, add the following solution to the medium: 

100	ml	**Phosphate buffer**	


## Phosphate buffer

5.44	g	KH2PO4	
43	g	Na2HPO4 x 12 H2O	
1000	ml	Distilled water	

1. Adjust pH to 7.2


## Nitsch's trace elements

0.5	ml	H2SO4	
2.2	g	MnSO4 x n H2O	
0.5	g	ZnSO4 x 7 H2O	
0.5	g	H3BO3	
0.016	g	CuSO4 x 5 H2O	
0.025	g	Na2MoO4 x 2 H2O	
0.046	g	CoCl2 x 6 H2O	
1000	ml	Distilled water