# J1047: MARINITOGA MEDIUM


## Main sol. J1047

30	g	Sea Salt (Sigma)	
6	g	PIPES	
1	g	Yeast extract	
1	g	Tryptone (BD-Difco)	
0.5	mg	Resazurin	
1000	ml	Distilled water	

1. Mix components and adjust pH to 7.0. Bubble the medium with N2 for 5 min, then distribute the medium into culture vessels under N2, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions aseptically and anaerobically (autoclaved and stored under a N2 gas atmosphere): 

14	ml	Glucose (1.0 M)	
10	ml	Na2S x 9 H2O (5%)	
10	ml	L-Cysteine HCl x H2O (5%)